Method for screening antimicrobial gels against multi-species oral biofilms

Kanchana Chathoth; Bénédicte Martin; Martine Bonnaure-Mallet; Christine Baysse

doi:10.1016/j.mimet.2021.106253

Method for screening antimicrobial gels against multi-species oral biofilms

J Microbiol Methods. 2021 Aug:187:106253. doi: 10.1016/j.mimet.2021.106253. Epub 2021 Jun 1.

Authors

Kanchana Chathoth¹, Bénédicte Martin¹, Martine Bonnaure-Mallet², Christine Baysse³

Affiliations

¹ University of Rennes 1, NUMECAN INSERM U1241, CIMIAD, F-35043 Rennes, France.
² University of Rennes 1, NUMECAN INSERM U1241, CIMIAD, F-35043 Rennes, France; CHU Pontchaillou, Rennes, France.
³ University of Rennes 1, NUMECAN INSERM U1241, CIMIAD, F-35043 Rennes, France. Electronic address: christine.baysse@univ-rennes1.fr.

PMID: 34087262
DOI: 10.1016/j.mimet.2021.106253

Abstract

We described a microtiter plate-based method that was effectively tailored for testing gel formulations against oral multispecies biofilms established on peg-lids. This method lifts the limitations imposed mainly by the anaerobic nature of the targeted bacterial species and the viscous properties of the targeted treatments.

Keywords: Antibacterial gels; Hyaluronic acid; MBEC™ assay; Oral biofilm; Oxygen; Porphyromonas gingivalis; Streptococcus gordonii; Treponema denticola.

MeSH terms

Anti-Bacterial Agents / pharmacology*
Bacteria / drug effects*
Bacteria / growth & development
Biofilms / drug effects*
Biofilms / growth & development
Gels
Humans
Microbial Sensitivity Tests / methods*
Mouth / microbiology*
Porphyromonas gingivalis / drug effects
Porphyromonas gingivalis / growth & development
Streptococcus gordonii / drug effects
Streptococcus gordonii / growth & development
Treponema denticola / drug effects
Treponema denticola / growth & development

Substances

Anti-Bacterial Agents
Gels