Make microalgal cultures axenic again - a fast and simple workflow utilizing fluorescence-activated cell sorting

Philipp Doppler; Ricarda Kriechbaum; Benedikt Singer; Oliver Spadiut

doi:10.1016/j.mimet.2021.106256

Make microalgal cultures axenic again - a fast and simple workflow utilizing fluorescence-activated cell sorting

J Microbiol Methods. 2021 Jul:186:106256. doi: 10.1016/j.mimet.2021.106256. Epub 2021 May 31.

Authors

Philipp Doppler¹, Ricarda Kriechbaum², Benedikt Singer³, Oliver Spadiut⁴

Affiliations

¹ Research Division Biochemical Engineering, Institute of Chemical, Environmental and Bioscience Engineering, Technische Universität Wien, Gumpendorfer Strasse 1a, 1060 Vienna, Austria. Electronic address: philipp.doppler@tuwien.ac.at.
² Research Division Biochemical Engineering, Institute of Chemical, Environmental and Bioscience Engineering, Technische Universität Wien, Gumpendorfer Strasse 1a, 1060 Vienna, Austria. Electronic address: ricarda.kriechbaum@tuwien.ac.at.
³ Research Division Biochemical Engineering, Institute of Chemical, Environmental and Bioscience Engineering, Technische Universität Wien, Gumpendorfer Strasse 1a, 1060 Vienna, Austria.
⁴ Research Division Biochemical Engineering, Institute of Chemical, Environmental and Bioscience Engineering, Technische Universität Wien, Gumpendorfer Strasse 1a, 1060 Vienna, Austria. Electronic address: oliver.spadiut@tuwien.ac.at.

PMID: 34082050
DOI: 10.1016/j.mimet.2021.106256

Abstract

Since the removal of contaminations in microalgal cultures is extremely laborious and time-consuming, we developed a rapid workflow to obtain axenicity by a combination of fluorescence-activated cell sorting (FACS) and plate spreading. During method development, several cyanobacteria and green algae strains were successfully made axenic. At the end, method transferability to another FACS device was demonstrated. Our workflow offers great time-savings with less hands-on laboratory work compared to conventional isolation techniques.

Keywords: Axenization; Chlorophyll fluorescence; Cyanobacteria; FACS; Green algae; Isolation workflow.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Axenic Culture / instrumentation
Axenic Culture / methods*
Cyanobacteria / growth & development
Cyanobacteria / isolation & purification
Flow Cytometry / methods*
Microalgae / cytology
Microalgae / growth & development*
Workflow