Quantitatively Controlled Intercellular Mitochondrial Transfer by Cell Fusion-Based Method Using a Microfluidic Device

Methods Mol Biol. 2021:2277:39-47. doi: 10.1007/978-1-0716-1270-5_3.

Abstract

Quantitative control of mitochondrial transfer is a promising approach for genetic manipulation of mitochondrial DNA (mtDNA) because it enables precise modulation of heteroplasmy. Furthermore, single mitochondrion transfer from a mtDNA mutation-accumulated cell to a mtDNA-less (ρ0) cell potentially achieves homoplasmy of mutated mtDNA. Here we describe the method for quantitative control of mitochondrial transfer including achieving single mitochondrion transfer between live single cells using a microfluidic device.

Keywords: Cell fusion; Heteroplasmy; Homoplasmy; Microfluidics; Microtunnel; Mitochondrial DNA; Mitochondrial transfer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Fusion*
  • Cytological Techniques / instrumentation
  • Cytological Techniques / methods*
  • DNA, Mitochondrial / genetics
  • Equipment Design
  • Humans
  • Lab-On-A-Chip Devices*
  • Mitochondria / genetics*
  • Mutation

Substances

  • DNA, Mitochondrial