Methyl jasmonate (MeJA) is an endogenous plant hormone, which plays an important role in agriculture production. A novel probe-free electrochemical immunosensor was fabricated for detecting of MeJA. Fc, carboxylated graphene (COOH-GR) and carboxylated multi-walled carbon nanotubes (COOH-MWNT) composite was formed and used to fabricate screen-printed electrode (SPE). Fc was used as the electronic medium. COOH-GR and COOH-MWNT were used to improve the conductivity and catalytic activity of the sensor and to immobilize the MeJA antibody. Thus, the immunosensor can be used to detect MeJA without external redox probe solution. The designed sensor can detect MeJA in a wide range of 100 fM-100 μM, and its detection limit is as low as 31.26 fM (S/N = 3). The as-prepared probe-free immunosensor is simple, low cost, and does not need redox probe solutions for measurements, which shows great promise for future application in precision agriculture.
Keywords: Electrochemical; Ferrocene; Immunosensor; Methyl jasmonate; Probe-free.
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