The utilization of pesticides has been increased in recent years due to population growth and increasing urbanization. The constant use of pesticides has resulted in contamination of the environment and agricultural products with serious human health concerns associated with their use. Therefore, detection and quantification of pesticides by sensitive and selective methods is highly required in food safety management. Traditional detection methods cannot realize highly sensitive, selective and on-site detection, which limits their application. (Bio)sensors and (bio)assays are emerging tools with unique properties such as rapid, sensitive, efficient and portable detection. Among them, enzyme-based biosensors have been widely developed and some have even been commercialized. However, they suffer from some limitations such as instability and low reproducibility that originate from the nature of enzyme. Non-enzymatic (bio)sensors overcome the current limitations of enzyme-based detection methods and provide great potential for efficient, highly sensitive and low-cost detection assays using smart and miniaturized devices. In this study, we provide an overview of recent advances and new trends in optical and electrochemical non-enzymatic (bio)sensors for the detection of pesticides by focusing on antibody, aptamer and molecularly imprinted polymer (MIP) as recognition elements. Performance, advantages and drawbacks of the developed (bio)sensors are discussed well. The main advantage these recognition elements is their stability over an extended period of time compared to the enzymes. Furthermore, the combination of nanomaterials in these (bio)sensors can significantly improve their performance.
Keywords: (Bio)sensor; (Bio)sssay; Antibody; Aptamer; Molecularly imprinted polymer; Pesticide.
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