Cytotoxicity and Nitric Oxide Production Inhibitory Activities of Compounds Isolated from the Plant Pathogenic Fungus Curvularia sp

Virayu Suthiphasilp; Achara Raksat; Tharakorn Maneerat; Sarinya Hadsadee; Siriporn Jungsuttiwong; Stephen G Pyne; Putarak Chomnunti; Wuttichai Jaidee; Rawiwan Charoensup; Surat Laphookhieo

doi:10.3390/jof7060408

Cytotoxicity and Nitric Oxide Production Inhibitory Activities of Compounds Isolated from the Plant Pathogenic Fungus Curvularia sp

J Fungi (Basel). 2021 May 22;7(6):408. doi: 10.3390/jof7060408.

Authors

Affiliations

¹ Center of Chemical Innovation for Sustainability, School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand.
² Medicinal Plants Innovation Center of Mae Fah Luang University, Chiang Rai 57100, Thailand.
³ Center for Organic Electronic and Alternative Energy, Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Ubon Ratchathani University, Ubon Ratchathani 34190, Thailand.
⁴ School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW 2522, Australia.
⁵ Center of Excellence in Fungal Research, School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand.
⁶ School of Integrative Medicine, Mae Fah Luang University, Chiang Rai 57100, Thailand.

Abstract

Chemical investigation of the mycelia of the pathogenic fungus Curvularia sp. which was isolated from a leaf of Dactyloctenium aegyptium (crowfoot grass), resulted in the isolation of a new compound, curvulariahawadride (5), along with five known compounds (1-4, and 6). Their structures were determined on the basis of spectroscopic data, including 1D and 2D NMR and HRESIMS. The absolute configuration of 5 was established from experimental and calculated electronic circular dichroism (ECD). Compounds 1, 3, and 5 showed nitric oxide (NO) production inhibitory activity with IC₅₀ values of 53.7, 32.8, and 12.8 µM, respectively. Compounds 2 and 4 showed significant cytotoxicity against lung cancer A549, colorectal cancer SW480, and leukemic K562 cells with an IC₅₀ ranging value of 11.73 to 17.59 µM.

Keywords: Curvularia sp.; Dactyloctenium aegyptium; curvulariahawadride; cytotoxicity; nitric oxide production inhibitory activity.

Abstract

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