Fibroblast functionality on novel Ti30Ta nanotube array

Patricia Capellato; Barbara S Smith; Ketul C Popat; Ana P R Alves Claro

doi:10.1016/j.msec.2012.05.013

Fibroblast functionality on novel Ti30Ta nanotube array

Mater Sci Eng C Mater Biol Appl. 2012 Oct 1;32(7):2060-2067. doi: 10.1016/j.msec.2012.05.013. Epub 2012 May 26.

Authors

Patricia Capellato¹, Barbara S Smith², Ketul C Popat³, Ana P R Alves Claro⁴

Affiliations

¹ Department of Materials, Faculty of Engineering Guaratinguetá, Sao Paulo State University-UNESP, Av. Ariberto Pereira da Cunha, 333, Pedregulho, CEP 12516-410, Guaratinguetá, SP, Brazil. Electronic address: pat_capellato@yahoo.com.br.
² School of Biomedical Engineering, Colorado State University, Fort Collins CO 80523, USA.
³ School of Biomedical Engineering, Colorado State University, Fort Collins CO 80523, USA; Department of Mechanical Engineering, Colorado State University, Fort Collins, CO 80523, USA.
⁴ Department of Materials, Faculty of Engineering Guaratinguetá, Sao Paulo State University-UNESP, Av. Ariberto Pereira da Cunha, 333, Pedregulho, CEP 12516-410, Guaratinguetá, SP, Brazil.

PMID: 34062696
DOI: 10.1016/j.msec.2012.05.013

Abstract

In this study, the mechanical substrate and topographical surface properties of anodized Ti30Ta alloy were investigated using scanning electron microscopy (SEM), energy-dispersive spectroscopy (EDS) and contact angle measurement. The anodization process was performed in an electrolyte solution containing HF (48%) and H₂SO₄ (98%) in the volumetric ratios 1:9 with the addition of 5% dimethyl sulfoxide (DMSO) at 15V, 25V and 35V for 20 and 40min, producing a nanotube architecture when anodized at 35V for 40min. Human dermal fibroblasts (HDF, neonatal) were utilized to evaluate the biocompatibility of Ti30Ta nanotubes and Ti30Ta alloy after 1 and 3days of culture. Cellular adhesion, proliferation, viability, cytoskeletal organization and morphology were investigated using fluorescence microscope imaging, biochemical assay and SEM imaging respectively. The results presented identify altered material properties and improved cellular interaction on Ti30Ta nanotubes as compared to Ti30 Ta alloy.

Keywords: Anodization; Human dermal fibroblasts; Ti30Ta nanotubes.