Heparin conjugated silica nanoparticle synthesis

Brigida Silvestri; Alessandro Pezzella; Giuseppina Luciani; Aniello Costantini; Fabiana Tescione; Francesco Branda

doi:10.1016/j.msec.2012.05.018

Heparin conjugated silica nanoparticle synthesis

Mater Sci Eng C Mater Biol Appl. 2012 Oct 1;32(7):2037-2041. doi: 10.1016/j.msec.2012.05.018. Epub 2012 Jun 2.

Authors

Brigida Silvestri¹, Alessandro Pezzella², Giuseppina Luciani³, Aniello Costantini³, Fabiana Tescione³, Francesco Branda³

Affiliations

¹ Department of Materials and Production Engineering, University of Naples Federico II, 80125, Naples, Italy. Electronic address: brigida.silvestri@unina.it.
² Department of Organic Chemistry and Biochemistry, University of Naples Federico II, 80126, Naples, Italy.
³ Department of Materials and Production Engineering, University of Naples Federico II, 80125, Naples, Italy.

PMID: 34062692
DOI: 10.1016/j.msec.2012.05.018

Abstract

We report on a facile method towards a synthesis of novel hybrid heparin silica nanoparticles involving a modification of the Stober method. Tetrapropyl orthosilicate (TPOS) was used instead of the traditional TEOS or TMOS; by this way we could overcome the solubility problems of heparin in ethanol and exploit the good solubility of heparin in proper isopropanol-water mixtures. Aminopropyl triethoxysilane (APTS) was also used to have a good link of heparin to silica particles. SEM, DLS, FTIR and NMR proved that we did find conditions in which heparin conjugated silica particles were produced. Thermogravimetry allowed to evaluate the heparin/silica weight ratio to be 0.61. The efficiency of heparin binding to the particles was appreciated to be 35wt.%.

Keywords: Heparin; Silica nanoparticles; Sol–gel method.