Decellularized (DC) kidney scaffold shows great potential for renal recovering. Our study explored the effect of the DC kidney scaffolds treating on chronic renal failure (CRF) through grafting them on 5/6 nephrectomized (5/6 Nx) rat kidneys compared with gelatin sponges covered the incision edges. Blood urea nitrogen and angiotensin II were significantly lower in most time in scaffold-grafted groups. Remnant kidney tumor necrosis factor-α and fibroblast growth factor in scaffold-grafted groups significantly reduced in majority of time points compared with controls. But platelet-derived growth factor-BB showed a different varied tendency, first higher in scaffold groups on week 2, 4, 6, but lower on week 8, finally no difference on week 12 compared with gelatin-sponge groups. In addition, the index of glomerular sclerosis was significantly lesser in scaffold-grafted groups, and, the accumulation of collagen III and collagen IV decreased in scaffold-grafted groups on week 6, 8, 12 compared with gelatin-sponge groups. Moreover, DC scaffolds enhanced the expression of CD133 on week 2, 6, 8, 12. In conclusion, DC kidney scaffold altered the healing response after 5/6 nephrectomy and ameliorated renal injury to some degree. Therefore, DC kidney scaffold could be a promising therapeutic method on CRF.
Keywords: 5/6 nephrectomized; CD133; chronic renal failure; decellularized kidney scaffold; renal recovery.
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