Bacillus fonticola sp. nov., isolated from deep sea cold seep sediment

Abstract

In this study, we report a novel Gram-positive bacterium, designated as strain CS13^T, isolated from deep-sea sediment collected in the cold seep area of the South China Sea. Growth of strain CS13^T occurred at 16-37 °C (optimum 25-28 °C), pH 7.0-9.0 (optimum, 7.0), and 0-8% (w/v) NaCl (optimum, 2-3%). Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain CS13^T belonged to the genus Bacillus. The closest phylogenetic neighbors of strain CS13^T are Bacillus carboniphilus JCM 9731^T (96.0%), Bacillus pakistanensis NCCP-168^T (95.7%) and Bacillus acidicola 105-2^T (95.6%). The genomic DNA G + C content of strain CS13^T is 43.7 mol%. The principal respiratory quinone was menaquinone 7 (MK-7). The polar lipids of CS13^T contained diphosphatidylglycerol, phosphatidylglycerol, phospholipid, and glycolipid. The major fatty acids of CS13^T contained anteiso-C_15:0, anteiso-C_17:0, C_16:0 and C_18:0. Strain CS13^T harboured meso-diaminopimelic acid as the diagnostic diamino acid. Phylogenetic, physiological, biochemical, and morphological analyses suggested that strain CS13^T represents a novel species of genus Bacillus, and the name Bacillus fonticola sp. nov. is proposed for the type species CS13^T (= CCTCC AB 2019194^T = JCM 33663^T).

Keywords: Bacillus; Cold seep; Deep sea; South China sea.