A Novel Mechanical-Based Injective Hydrogel for Treatment with Aromatase Inhibitors Caused Joint Inflammation via the NF-κB Pathway

Zipeng Yang; Chang-Peng Xu; Yuhui Chen; Wenqiang Li; Liping Wang; Zi-Guo Yuan

doi:10.1021/acsomega.1c00580

A Novel Mechanical-Based Injective Hydrogel for Treatment with Aromatase Inhibitors Caused Joint Inflammation via the NF-κB Pathway

ACS Omega. 2021 Apr 10;6(15):10242-10249. doi: 10.1021/acsomega.1c00580. eCollection 2021 Apr 20.

Authors

Zipeng Yang¹, Chang-Peng Xu², Yuhui Chen³, Wenqiang Li⁴, Liping Wang⁵, Zi-Guo Yuan¹

Affiliations

¹ Laboratory of Parasitology College of Veterinary Medicine, South China Agricultural University, Guangzhou 510000, China.
² Department of Orthopaedics, Guangdong Second Provincial General Hospital, Guangzhou, Guangdong 510317, P.R. China.
³ Orthopedic Hospital of Guangdong Province, Department of Orthopedic Surgery, The Third Affiliated Hospital of Southern Medical University, Guangzhou 510630, China.
⁴ Guangzhou Sport University, Guangzhou 510500, China.
⁵ UniSA Cancer Research Institute, UniSA Clinical & Health Sciences, University of South Australia, Adelaide, SA 5001, Australia.

Abstract

Synovium has widely participated in induced inflammation, suggesting that it is a potential target to reduce aromatase inhibitors (AIs) causing joint inflammation or pain. Exercise and mechanical stimulation are important strategies for precaution and treatment of bone inflammation. In this work, we developed a novel thermo-sensitive hydrogel, which could be injected intra-articularly. The aim of this research was to investigate the role of various mechanical strength hydrogels in reducing synovium inflammation. The effect of different mechanical strength hydrogels on regulating synovium inflammation was used to stimulate human fibroblast-like synoviocytes (FLS) under a cyclic mechanical compression environment in vitro. Cytokine and metalloprotease expression in FLS was analyzed by the western blot and q-PCR method, in which FLS were cultured with the different mechanical strength hydrogels. The results showed that a moderate-intensity hydrogel mechanical stimulation might be suitable in reducing AI-induced FLS inflammation via the NK-κB pathway. In addition, we built an AI-treated rat model and injected the different mechanical strength hydrogels. Similarly, the moderate-strength mechanical hydrogel could reduce the inflammatory factor and metalloproteinase expression in synovial tissues and intra-articular synovia.