LncRNA MSC-AS1 Promotes Colorectal Cancer Progression by Regulating miR-325/TRIM14 Axis

Changhong He; Xia Wang; Meichun Du; Yanjun Dong

doi:10.1155/2021/9954214

LncRNA MSC-AS1 Promotes Colorectal Cancer Progression by Regulating miR-325/TRIM14 Axis

J Oncol. 2021 May 11:2021:9954214. doi: 10.1155/2021/9954214. eCollection 2021.

Authors

Changhong He¹, Xia Wang², Meichun Du³, Yanjun Dong⁴

Affiliations

¹ Department of Clinical Laboratory, Yantaishan Hospital, Yantai 264000, China.
² Blood Purification Centre, East Hospital, Qingdao Municipal Hospital, Qingdao 266071, China.
³ PICC Clinic, Qingdao Central Hospital Affiliated to Qingdao University, Qingdao 266042, China.
⁴ Department of Clinical Laboratory, People's Hospital of Rizhao, Rizhao 276800, China.

Abstract

Background: LncRNA MSC-AS1 has been reported to be a tumor promoter in hepatocellular carcinoma. However, the function of MSC-AS1 in colorectal cancer (CRC) has not been elucidated. It is designed to study the expression level of MSC-AS1 and investigate its biological effect on the progression of CRC.

Methods: The expression patterns of MSC-AS1, miR-325, and TRIM14 were explored by RT-qPCR in CRC tissues and cells. The protein expression of TRIM14 was tested by Western blot assay. The association between MSC-AS1 expression and clinicopathological data was analyzed by chi-squared test. CCK-8 assay, colony formation, and Transwell assay were used to investigate the effect of MSC-AS1 on cell growth, invasion, and migration in CRC cells. The correlations among MSC-AS1, miR-325, and TRIM14 were analyzed by Pearson's correlation coefficient analysis.

Results: We found that MSC-AS1 and TRIM14 were upregulated in CRC tissues, while miR-325 was downregulated in CRC tissues. Functional experiments demonstrated that MSC-AS1 knockdown inhibited cell proliferation, migration, and invasion abilities in CRC cells. Additionally, miR-325 was proved to be a target miRNA of MSC-AS1, and TRIM14 might be a downstream gene of miR-325. Besides that, MSC-AS1 counteracted the inhibitory effect of miR-325 on the cell progression and TRIM14 expression.

Conclusion: Our results indicated that MSC-AS1 facilitated CRC progression by sponging miR-325 to upregulate TRIM14 expression. We suggested that MSC-AS1 might be a potential lncRNA-target for CRC therapy.