Characterization of chilled chicken spoilage using an integrated microbiome and metabolomics analysis

Tao Zhang; Hao Ding; Lan Chen; Shanshan Zhang; Pengfei Wu; Kaizhou Xie; Zhiming Pan; Genxi Zhang; Guojun Dai; Haiqing Wu; Jinyu Wang

doi:10.1016/j.foodres.2021.110328

Characterization of chilled chicken spoilage using an integrated microbiome and metabolomics analysis

Food Res Int. 2021 Jun:144:110328. doi: 10.1016/j.foodres.2021.110328. Epub 2021 Mar 27.

Authors

Tao Zhang¹, Hao Ding², Lan Chen³, Shanshan Zhang⁴, Pengfei Wu⁵, Kaizhou Xie⁶, Zhiming Pan⁷, Genxi Zhang⁸, Guojun Dai⁹, Haiqing Wu¹⁰, Jinyu Wang¹¹

Affiliations

¹ College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education, Yangzhou University, Yangzhou 225009, China; Jiangsu Key Laboratory of Zoonosis, Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Yangzhou University, Yangzhou 225009, China. Electronic address: zhangt@yzu.edu.cn.
² College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education, Yangzhou University, Yangzhou 225009, China. Electronic address: 15138343214@163.com.
³ College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education, Yangzhou University, Yangzhou 225009, China; College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China. Electronic address: chenlan9326@163.com.
⁴ College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education, Yangzhou University, Yangzhou 225009, China. Electronic address: zss18352764956@163.com.
⁵ College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education, Yangzhou University, Yangzhou 225009, China. Electronic address: wu_p_fei@163.com.
⁶ College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education, Yangzhou University, Yangzhou 225009, China. Electronic address: kzxie@yzu.edu.cn.
⁷ Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education, Yangzhou University, Yangzhou 225009, China; Jiangsu Key Laboratory of Zoonosis, Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Yangzhou University, Yangzhou 225009, China. Electronic address: zmpan@yzu.edu.cn.
⁸ College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education, Yangzhou University, Yangzhou 225009, China. Electronic address: gxzhang@yzu.edu.cn.
⁹ College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education, Yangzhou University, Yangzhou 225009, China. Electronic address: daigj@yzu.edu.cn.
¹⁰ College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education, Yangzhou University, Yangzhou 225009, China. Electronic address: hqwu@yzu.edu.cn.
¹¹ College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education, Yangzhou University, Yangzhou 225009, China. Electronic address: jywang@yzu.edu.cn.

PMID: 34053532
DOI: 10.1016/j.foodres.2021.110328

Abstract

Spoilage of chilled chicken can occur as a result of microbial development and consumption of meat nutrients by spoilage bacteria, ultimately resulting in the release of undesired metabolites. Characterizing the profiles of the microbiota and metabolites and clarifying their relationships will contribute to an improved understanding of the mechanism underlying chilled chicken spoilage. In the present study, 16S rRNA gene sequencing and ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS)-based untargeted metabolomics analyses were applied to determine the microbial and metabolic profiles in chicken during chilled storage. The microbial and metabolic datasets were subjected to combined analysis using weighted gene co-expression network analysis (WGCNA) and Spearman's correlation analysis. Brochothrix, Carnobacterium, Photobacterium, Pseudomonas, Acinetobacter, Serratia, Kurthia, Shewanella, and Obesumbacterium genera were identified as the dominant spoilage bacteria in chilled chicken. Ten metabolic pathways, including histidine metabolism and purine metabolism, were identified as potential mechanisms underlying chilled chicken spoilage. Correlation analysis demonstrated that spoilage bacterial genera were highly correlated with spoilage-related metabolites. Taken together, the present study proposed an integrated microbiome and metabolomics approach to investigate the mechanism of chilled chicken spoilage caused by microbial activity. The results obtained by this approach provide a comprehensive insight into changes in the microbial and metabolic profiles of chilled chicken during spoilage.

Keywords: Chilled chicken; Metabolic profiles; Metabolomics; Microbiome; Spoilage microbiota.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Chickens*
Food Microbiology
Metabolomics
Microbiota*
RNA, Ribosomal, 16S
Tandem Mass Spectrometry

Substances

RNA, Ribosomal, 16S