High-throughput quantification of eighteen heterocyclic aromatic amines in roasted and pan-fried meat on the basis of high performance liquid chromatography-quadrupole-orbitrap high resolution mass spectrometry

Yan Xu; Haixia Li; Jian Liang; Jina Ma; Juan Yang; Xiaojuan Zhao; Wenhong Zhao; Weidong Bai; Xiaofang Zeng; Hao Dong

doi:10.1016/j.foodchem.2021.130147

High-throughput quantification of eighteen heterocyclic aromatic amines in roasted and pan-fried meat on the basis of high performance liquid chromatography-quadrupole-orbitrap high resolution mass spectrometry

Food Chem. 2021 Nov 1:361:130147. doi: 10.1016/j.foodchem.2021.130147. Epub 2021 May 18.

Authors

Yan Xu¹, Haixia Li¹, Jian Liang², Jina Ma², Juan Yang¹, Xiaojuan Zhao¹, Wenhong Zhao¹, Weidong Bai¹, Xiaofang Zeng³, Hao Dong⁴

Affiliations

¹ College of Light Industry and Food Sciences, Guangdong Key Laboratory of Science and Technology of Lingnan Special Food, Innovation Research Institute of Modern Agricultural Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
² Guangzhou Highgoal Biotech Company Limited, Guangzhou 510110, China.
³ College of Light Industry and Food Sciences, Guangdong Key Laboratory of Science and Technology of Lingnan Special Food, Innovation Research Institute of Modern Agricultural Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China. Electronic address: xiaofang_zeng2015@163.com.
⁴ College of Light Industry and Food Sciences, Guangdong Key Laboratory of Science and Technology of Lingnan Special Food, Innovation Research Institute of Modern Agricultural Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China. Electronic address: donghao@zhku.edu.cn.

PMID: 34051597
DOI: 10.1016/j.foodchem.2021.130147

Abstract

Triple quadrupole mass spectrometry has been the main technique for HAAs analysis in recent decade, while it requires extensive optimization of compound-dependent parameters. A novel method based on HPLC-Q-Orbitrap-HRMS was developed firstly for simultaneous determination of eighteen HAAs. Extraction and purification conditions were optimized and the developed method was validated in terms of linearity, accuracy and precision. Results indicated eighteen HAAs and two internal standards could be separated in 12 min using a gradient elution program. The full MS/dd-MS² scan was adopted for analysis, which indicated favorable recoveries (71.3-114.8%) along with LODs and LOQs in the ranges of 0.02-0.6 and 0.05-2.0 μg/kg, respectively. Internal standards used for calibration could effectively reduce quantification errors produced by matrix effects. The validated method was successfully applied for HAAs analysis in roasted and pan-fried meat and was confirmed to be an alternative method when triple quadrupole mass spectrometry is absent in lab.

Keywords: Heterocyclic aromatic amine; Liquid chromatography; Orbitrap; Pan-fried beef patty; Roasted beef patty.

MeSH terms

Amines / analysis*
Amines / chemistry
Calibration
Chromatography, High Pressure Liquid / methods*
Cooking
Food Analysis / methods
Heterocyclic Compounds / analysis*
Heterocyclic Compounds / chemistry
Limit of Detection
Meat / analysis*
Reproducibility of Results
Tandem Mass Spectrometry / methods*

Substances

Amines
Heterocyclic Compounds