Emergence of blaNDM-9-bearing tigecycline-resistant Klebsiella aerogenes of chicken origin

J Glob Antimicrob Resist. 2021 Sep:26:66-68. doi: 10.1016/j.jgar.2021.04.028. Epub 2021 May 26.

Abstract

Objectives: The aim of this study was to characterise a tigecycline-resistant blaNDM-9-bearing Klebsiella aerogenes strain (HNHF1) of chicken origin.

Methods: Strain HNHF1 was characterised by phenotypic antimicrobial susceptibility testing, PCR, conjugation assays, S1 nuclease pulsed-field gel electrophoresis (S1-PFGE), whole-genome sequencing and bioinformatics analysis.

Results: The blaNDM-9 gene was located on an IncHI2 plasmid (pHNHF1_NDM-9) carrying various antimicrobial resistance genes. Moreover, the genetic context ΔISAba125-blaNDM-9-bleMBL-trpF is similar to other blaNDM-bearing genetic contexts. TA cloning experiments showed that tet(A) variants may play a partial role in high-level tigecycline resistance in HNHF1.

Conclusion: This is the first report of a tigecycline-resistant blaNDM-9-bearing IncHI2 plasmid in a K. aerogenes ST4 isolate of animal origin, which poses a great threat to public health. Further comprehensive surveillance is needed.

Keywords: Animal origin; Carbapenem resistance; Plasmid; bla(NDM-9).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chickens
  • Enterobacter aerogenes* / genetics
  • Klebsiella pneumoniae / genetics
  • Multilocus Sequence Typing
  • Tigecycline / pharmacology
  • beta-Lactamases / genetics

Substances

  • Tigecycline
  • beta-Lactamases