Simultaneous Detection of Four Cell Cycle Phases with Live Fluorescence Imaging

Bryce T Bajar; Michael Z Lin

doi:10.1007/978-1-0716-1258-3_3

Simultaneous Detection of Four Cell Cycle Phases with Live Fluorescence Imaging

Methods Mol Biol. 2021:2274:25-35. doi: 10.1007/978-1-0716-1258-3_3.

Authors

Bryce T Bajar¹, Michael Z Lin^{2

3}

Affiliations

¹ Department of Biological Chemistry, Medical Scientist Training Program, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, USA.
² Department of Neurobiology, Stanford University, Stanford, CA, USA. mzlin@stanford.edu.
³ Department of Bioengineering, Stanford University, Stanford, CA, USA. mzlin@stanford.edu.

PMID: 34050459
DOI: 10.1007/978-1-0716-1258-3_3

Abstract

Visualizing progression through the cell cycle provides valuable information for the study of development, tissue maintenance, and dysregulated growth in proliferative diseases, such as cancer. Developments in fluorescent biosensors have facilitated dynamic tracking of molecular processes, including the cell cycle. The genetically encoded set of fluorescent indicators, Fucci4, enables the visualization of transitions between each cell cycle phase. Here, we describe a method to track progression through each cell cycle phase using Fucci4 in live epifluorescence imaging. In principle, this approach can be adapted to in vitro time-lapse imaging of any four spectrally resolvable fluorescent indicators.

Keywords: Cell cycle; Fluorescence microscopy; Genetically encoded fluorescent indicator; Live-cell imaging; Multichannel imaging.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Biosensing Techniques / methods*
Cell Cycle*
Cell Division
Fluorescence*
HeLa Cells
Humans
Luminescent Proteins / metabolism*
Microscopy, Fluorescence / methods*
Optical Imaging / methods*

Substances

Luminescent Proteins

Abstract

Publication types

MeSH terms

Substances

Grants and funding