Accessible and Validated Processing of SARS-CoV-2 from Wastewater

Nicole C Rondeau; Oliver J Rose; Lina A Ariyan; Brian J Mailloux; Jj L Miranda

doi:10.1128/MRA.00174-21

Accessible and Validated Processing of SARS-CoV-2 from Wastewater

Microbiol Resour Announc. 2021 May 27;10(21):e0017421. doi: 10.1128/MRA.00174-21. Epub 2021 May 27.

Authors

Nicole C Rondeau¹, Oliver J Rose², Lina A Ariyan¹, Brian J Mailloux³, Jj L Miranda¹

Affiliations

¹ Department of Biology, Barnard College, Columbia University, New York, New York, USA.
² Office of Facilities Services, Barnard College, Columbia University, New York, New York, USA.
³ Environmental Science Department, Barnard College, Columbia University, New York, New York, USA.

Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the etiological agent of coronavirus disease 2019 (COVID-19), is shed into wastewater. Accessible methods are necessary for processing samples in a myriad of contexts. We optimized a protocol for extracting viral RNA for downstream experiments. Our pipeline was validated with SARS-CoV-2 itself as a matrix recovery and quantitative measurement control.