Herein, a novel and sensitive fluorescence method for cartap determination is established on the basis of the inner filter effect (IFE) of gold nanoparticles (Au NPs) on the fluorescence of glutathione protected Cu NCs (GSH-Cu NCs). In the presence of Au NPs, the fluorescence of GSH-Cu NCs was strongly quenched by the IFE because the absorption spectra of Au NPs overlap well with the emission spectra of GSH-Cu NCs. Upon addition of cartap, cartap could induce the aggregation of Au NPs whose absorption spectrum does not overlap with the emission spectrum of GSH-Cu NCs. Then, with the increase in cartap concentration, the IFE-decreased fluorescence was gradually recovered, realizing the fluorescence sensing of cartap. Under optimal conditions, the proposed method has a good linear relationship with cartap concentration in the range of 7-100 nM, and the detection limit is 3.34 nM. In addition, satisfactory results were obtained for cartap analysis using tap water and cabbage as real samples, which demonstrated that the method as-developed would have great practical application prospects.