In vitro co-culture of human intestinal organoids and lamina propria-derived CD4+ T cells

Renée R C E Schreurs; Martin E Baumdick; Agata Drewniak; Madeleine J Bunders

doi:10.1016/j.xpro.2021.100519

In vitro co-culture of human intestinal organoids and lamina propria-derived CD4⁺ T cells

STAR Protoc. 2021 May 14;2(2):100519. doi: 10.1016/j.xpro.2021.100519. eCollection 2021 Jun 18.

Authors

Renée R C E Schreurs¹, Martin E Baumdick², Agata Drewniak^{1

3}, Madeleine J Bunders^{1

2}

Affiliations

¹ Department of Experimental Immunology, Amsterdam Infection & Immunity Institute, Amsterdam University Medical Center, University of Amsterdam, Amsterdam 1105 AZ, the Netherlands.
² Research Department Virus Immunology, Leibniz Institute for Experimental Virology, Hamburg 20251, Germany.
³ Kiadis Pharma B.V., Amsterdam 1105 BV, the Netherlands.

Abstract

Crosstalk between immune cells and intestinal stem cells (ISCs) in vivo plays a critical role in tissue homeostasis and inflammation; however, in vitro models based on primary cells recapitulating this interaction were lacking. Here, we provide a detailed protocol for an autologous in vitro long-term 3D co-culture system of human intestinal CD4⁺ T cells and ISCs to study T cell-intestinal epithelial cell interactions during tissue development and inflammation. For complete details on the use and execution of this protocol, please refer to Schreurs et al. (2019).

Keywords: Cell culture; Immunology; Organoids.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

CD4-Positive T-Lymphocytes / cytology
CD4-Positive T-Lymphocytes / immunology*
Coculture Techniques
Humans
Intestinal Mucosa / cytology
Intestinal Mucosa / immunology*
Organoids / cytology
Organoids / immunology*