Evaluation of Bioactivity of Strigolactone-Related Molecules by a Quantitative Luminometer Bioassay

Methods Mol Biol. 2021:2309:191-200. doi: 10.1007/978-1-0716-1429-7_15.

Abstract

The binding of strigolactones to their receptor, the α/β hydrolase DWARF14 (D14), leads to the modulation of transcriptional activity by destabilization of specific transcriptional corepressors via proteasomal degradation. Subsequently, strigolactones also promote D14 degradation by the same pathway. Here we describe an innovative quantitative bioassay based on Arabidopsis transgenic lines expressing AtD14 fused to the firefly luciferase, developed to identify new strigolactone analogs capable to activate the strigolactone signaling.

Keywords: Bioassay; DWARF14; Luciferase; Perception; Plant hormones; Strigolactones.

MeSH terms

  • Arabidopsis / drug effects*
  • Arabidopsis / genetics
  • Arabidopsis / metabolism
  • Arabidopsis Proteins / agonists*
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism
  • Biological Assay*
  • Genes, Reporter
  • Heterocyclic Compounds, 3-Ring / pharmacology*
  • Lactones / pharmacology*
  • Luciferases, Firefly / genetics
  • Luciferases, Firefly / metabolism
  • Luminescent Measurements
  • Plant Growth Regulators / pharmacology*
  • Plants, Genetically Modified / drug effects*
  • Plants, Genetically Modified / genetics
  • Plants, Genetically Modified / metabolism
  • Proteolysis
  • Receptors, Cell Surface / agonists*
  • Receptors, Cell Surface / genetics
  • Receptors, Cell Surface / metabolism
  • Recombinant Fusion Proteins / metabolism
  • Signal Transduction

Substances

  • Arabidopsis Proteins
  • DWARF14 protein, Arabidopsis
  • GR24 strigolactone
  • Heterocyclic Compounds, 3-Ring
  • Lactones
  • Plant Growth Regulators
  • Receptors, Cell Surface
  • Recombinant Fusion Proteins
  • Luciferases, Firefly