Protocol for derivation of organoids and patient-derived orthotopic xenografts from glioma patient tumors

Anaïs Oudin; Virginie Baus; Vanessa Barthelemy; Carina Fabian; Eliane Klein; Monika Dieterle; May Wantz; Ann-Christin Hau; Claire Dording; Amandine Bernard; Alessandro Michelucci; Yahaya A Yabo; Georgia Kanli; Olivier Keunen; Rolf Bjerkvig; Simone P Niclou; Anna Golebiewska

doi:10.1016/j.xpro.2021.100534

Protocol for derivation of organoids and patient-derived orthotopic xenografts from glioma patient tumors

STAR Protoc. 2021 May 12;2(2):100534. doi: 10.1016/j.xpro.2021.100534. eCollection 2021 Jun 18.

Authors

Anaïs Oudin¹, Virginie Baus¹, Vanessa Barthelemy¹, Carina Fabian^{1

2}, Eliane Klein¹, Monika Dieterle¹, May Wantz¹, Ann-Christin Hau¹, Claire Dording¹, Amandine Bernard^{1

3}, Alessandro Michelucci^{1

3}, Yahaya A Yabo^{1

4}, Georgia Kanli^{1

5

6}, Olivier Keunen^{1

5

6}, Rolf Bjerkvig^{1

2}, Simone P Niclou^{1

2}, Anna Golebiewska¹

Affiliations

¹ NORLUX Neuro-Oncology Laboratory, Department of Oncology, Luxembourg Institute of Health, 1526 Luxembourg, Luxembourg.
² Department of Biomedicine, University of Bergen, 5019 Bergen, Norway.
³ Neuro-Immunology Group, Department of Oncology, Luxembourg Institute of Health, 1526 Luxembourg, Luxembourg.
⁴ Faculty of Science, Technology and Medicine, University of Luxembourg, 4367 Belvaux, Luxembourg.
⁵ Quantitative Biology Unit, Luxembourg Institute of Health, 1445 Strassen, Luxembourg.
⁶ Translational Radiomics, Department of Oncology, Luxembourg Institute of Health, 1526 Luxembourg, Luxembourg.

Abstract

Tumor organoids and patient-derived orthotopic xenografts (PDOXs) are some of the most valuable pre-clinical tools in cancer research. In this protocol, we describe efficient derivation of organoids and PDOX models from glioma patient tumors. We provide detailed steps for organoid culture, intracranial implantation, and detection of tumors in the brain. We further present technical adjustments for standardized functional assays and drug testing. For complete details on the use and execution of this protocol, please refer to Golebiewska et al. (2020).

Keywords: Cancer; Cell Biology; Cell culture; Cell isolation; Model Organisms; Neuroscience; Organoids.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antineoplastic Agents / pharmacology
Brain Neoplasms / pathology*
Cell Culture Techniques
Drug Screening Assays, Antitumor / methods*
Female
Glioma / pathology*
Heterografts* / cytology
Heterografts* / drug effects
Humans
Male
Mice
Organoids* / cytology
Organoids* / drug effects
Tumor Cells, Cultured / cytology

Substances

Antineoplastic Agents