Biotechnological production of ssDNA with DNA-hydrolyzing deoxyribozymes

Jin Liu; Hongzhou Gu

doi:10.1016/j.xpro.2021.100531

Biotechnological production of ssDNA with DNA-hydrolyzing deoxyribozymes

STAR Protoc. 2021 May 10;2(2):100531. doi: 10.1016/j.xpro.2021.100531. eCollection 2021 Jun 18.

Authors

Jin Liu^{1

2}, Hongzhou Gu^{1

2}

Affiliations

¹ Fudan University Shanghai Cancer Center and the Shanghai Key Laboratory of Medical Epigenetics, Institutes of Biomedical Sciences, Shanghai Stomatological Hospital, Fudan University, Shanghai 200433, China.
² Center for Medical Research and Innovation, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai 201399, China.

Abstract

Preparation of long single-stranded (ss)DNA in large quantities with high efficiency and purity remains a synthetic challenge. Here, we present a protocol for using DNA-hydrolyzing DNA enzymes (deoxyribozymes) for efficient biotechnological production of milligrams of ssDNA with a customizable sequence up to a few kilobases. Our protocol provides a convenient yet economical way to store the sequence information of target ssDNA on phages for selective mass production on demand. For complete details on the use and execution of this protocol, please refer to Jia et al. (2021).

Keywords: atomic force microscopy (AFM); biotechnology and BIOENGINEERING; microbiology; molecular biology.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteriophages / genetics
Bioengineering
Biotechnology / methods*
DNA, Catalytic / metabolism*
DNA, Single-Stranded* / analysis
DNA, Single-Stranded* / genetics
DNA, Single-Stranded* / metabolism
Escherichia coli / genetics
Microscopy, Atomic Force

Substances

DNA, Catalytic
DNA, Single-Stranded