Transcriptional and Post-transcriptional Control of the Nitrate Respiration in Bacteria

Abstract

In oxygen (O₂) limiting environments, numerous aerobic bacteria have the ability to shift from aerobic to anaerobic respiration to release energy. This process requires alternative electron acceptor to replace O₂ such as nitrate (NO₃ ^-), which has the next best reduction potential after O₂. Depending on the organism, nitrate respiration involves different enzymes to convert NO₃ ^- to ammonium (NH₄ ⁺) or dinitrogen (N₂). The expression of these enzymes is tightly controlled by transcription factors (TFs). More recently, bacterial small regulatory RNAs (sRNAs), which are important regulators of the rapid adaptation of microorganisms to extremely diverse environments, have also been shown to control the expression of genes encoding enzymes or TFs related to nitrate respiration. In turn, these TFs control the synthesis of multiple sRNAs. These results suggest that sRNAs play a central role in the control of these metabolic pathways. Here we review the complex interplay between the transcriptional and the post-transcriptional regulators to efficiently control the respiration on nitrate.

Keywords: FNR; Hfq; denitrification; dissimilatory nitrate reduction; nitrate reductase; small RNAs; two-component systems.