Lung adenocarcinoma (LUAD) is the most common subtype of lung cancer and one of the main causes of cancer-related deaths. In the past decade, with the widespread use of computed tomography (CT) in routine screening for lung cancer, the incidence of LUAD presenting as small pulmonary nodules radiologically, has increased remarkably. The mechanisms of the occurrence and progression of LUADs are complex, and the prognoses of patients with LUAD vary significantly. Although significant progress has been made in targeted therapy and immunotherapy for LUADs in recent years, the drug resistance of tumor cells has not been effectively overcome, which limits the benefits of patients. With the accomplishment of the Human Genome Project, sequencing-based genomic and transcriptomics have come into the field of clinical and scientific researches. Single-cell sequencing, as a new type of sequencing method that has captured increasing attention recently, can perform specific analysis of cell populations at single-cell level, which can reveal the unique changes of each cell type. Single-cell sequencing can also provide accurate assessment on heterogeneous stromal cells and cancer cells, which is helpful to reveal the complexity of molecular compositions and differences between non- and malignant tissues. To sum up, it is an urgent need for clinicians and basic scientists to deeply understand the pathogenesis and development of LUAD, the heterogeneity of tumor microenvironment (TME) and the mechanism of drug resistance formation through single-cell sequencing, so as to discover new therapeutic targets. In this paper, we reviewed and summarized the application and progress in single-cell sequencing of LUADs. .
【中文题目：单细胞RNA测序在肺腺癌中的研究进展】 【中文摘要：肺腺癌（lung adenocarcinoma, LUAD）是临床上肺癌最常见的亚型，是癌症相关死亡最主要的原因之一。过去十几年中，随着薄层计算机断层扫描（computed tomography, CT）广泛用于常规肺癌筛查，影像学上表现为小结节的LUAD发病率显著增高，其发生发展机制复杂，个体预后差异显著。尽管近年来针对LUAD的靶向和免疫疗法取得了重大进展，但肿瘤细胞的耐药性始终未得到有效解决，从而限制了患者获益。随着人类基因组计划的完成，以测序为基本手段的基因组学及转录组学进入临床和科研人员的视野。单细胞测序作为近年来受到高度关注的新型测序手段，与二代测序相比，其能在单细胞水平上对细胞群体进行特异性分析，揭示出每种细胞类型独特的变化，在单细胞水平上对许多异质基质细胞和癌细胞进行较精准地评估，从而揭示了分子成分的复杂性以及与非恶性组织中相应成分的区别。综上，通过单细胞测序深入了解LUAD发生发展机制和肿瘤微环境（tumor microenvironment, TME）的异质性及其耐药性形成机制，从而发现新的治疗靶点是临床医生和基础科学家迫切的需求。本文综合论述了单细胞测序在LUAD中的具体应用和研究进展。 】 【中文关键词：肺肿瘤；单细胞测序；耐药性；演进】.
Keywords: Drug resistance; Lung neoplasms; Progression; Single-cell sequencing.