Cdc14 phosphatase downmodulates ESCRT-0 complex formation on vacuolar membranes and microautophagy after TORC1 inactivation

Tasnuva Sharmin; Shamsul Morshed; Most Naoshia Tasnin; Tsuneyuki Takuma; Takashi Ushimaru

doi:10.1016/j.bbrc.2021.05.021

Cdc14 phosphatase downmodulates ESCRT-0 complex formation on vacuolar membranes and microautophagy after TORC1 inactivation

Biochem Biophys Res Commun. 2021 Jul 5:561:158-164. doi: 10.1016/j.bbrc.2021.05.021. Epub 2021 May 21.

Authors

Tasnuva Sharmin¹, Shamsul Morshed¹, Most Naoshia Tasnin¹, Tsuneyuki Takuma², Takashi Ushimaru³

Affiliations

¹ Graduate School of Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, 422-8021, Japan.
² Course of Bioscience, Department of Science, Graduate School of Integrated Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, 422-8021, Japan.
³ Graduate School of Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, 422-8021, Japan; Course of Bioscience, Department of Science, Graduate School of Integrated Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, 422-8021, Japan. Electronic address: ushimaru.takashi@shizuoka.ac.jp.

PMID: 34023781
DOI: 10.1016/j.bbrc.2021.05.021

Abstract

Remodeling of vacuolar membranes mediated by endosomal sorting complex required for transport (ESCRT) is critical for microautophagy induction in budding yeast. Nutrient depletion and inactivation of target of rapamycin complex 1 (TORC1) protein kinase elicit recruitment of the ESCRT-0 complex (Vps27-Hse1) onto vacuolar membranes and ESCRT-mediated microautophagy induction. Mitotic protein phosphatase Cdc14 antagonizes TORC1-mediated phosphorylation in macroautophagy induction after nutrient starvation and TORC1 inactivation. Here, we report that Cdc14 downregulates microautophagy induction after TORC1 inactivation. Cdc14 dysfunction stimulated the vacuolar membrane recruitment of Hse1, but not Vps27, after TORC1 inactivation, promoting ESCRT-0 complex formation. Conversely, overexpression of CDC14 compromises Hse1 recruitment on vacuolar membranes and microautophagy induction after TORC1 inactivation. Thus, Cdc14 phosphatase regulates the fluxes of two types of autophagy in the opposite directions, namely, it elicits macroautophagy and attenuates microautophagy.

Keywords: Cdc14; ESCRT; Hse1; Microautophagy; TORC1; Vps27.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Cycle Proteins / metabolism*
Endosomal Sorting Complexes Required for Transport / metabolism*
Intracellular Membranes / metabolism*
Intracellular Membranes / pathology
Mechanistic Target of Rapamycin Complex 1 / metabolism*
Microautophagy
Phosphoric Monoester Hydrolases / metabolism
Protein Tyrosine Phosphatases / metabolism*
Receptors, Cytoplasmic and Nuclear / metabolism
Saccharomyces cerevisiae / metabolism*
Saccharomyces cerevisiae Proteins / metabolism*
Vacuoles / metabolism*
Vacuoles / pathology

Substances

CDC14 protein, S cerevisiae
Cell Cycle Proteins
Endosomal Sorting Complexes Required for Transport
Hse1 protein, S cerevisiae
Receptors, Cytoplasmic and Nuclear
Saccharomyces cerevisiae Proteins
VPS27 protein, S cerevisiae
Mechanistic Target of Rapamycin Complex 1
Phosphoric Monoester Hydrolases
Protein Tyrosine Phosphatases