Enhanced bioremediation of 2,3',4,4',5-pentachlorodiphenyl by consortium GYB1 immobilized on sodium alginate-biochar

Abstract

2,3',4,4',5-pentachlorodiphenyl (PCB 118), a dioxin-like PCB, is often detected in the environment and is difficult to be aerobically biodegraded. In this study, a novel polychlorinated biphenyl degrading consortium GYB1 that can metabolize PCB 118 was successfully obtained by acclimatization process. To enhance the application performance of free bacterial cells, consortium GYB1 was immobilized with sodium alginate and biochar to prepare SC-GYB1 beads. Orthogonal experiments indicated that the optimal composition of the beads (0.2 g) was 2.0% sodium alginate (SA) content, 2.0% wet weight of cells and 1.5% biochar content, which can degrade 50.50% PCB 118 in 5 d. Immobilization shortened the degradation half-life of 1 mg/L PCB 118 by consortium GYB1 from 8.14 d to 3.79 d and made the beads more robust to respond to environmental stress. The SC-GYB1 beads could even keep considerable PCB degradation ability under 200 mg/L Cd²⁺ stress. According to 16S rRNA gene analysis, Pseudomonas and Stenotrophomonas played the dominant role in consortium GYB1. And embedding obviously altered the community structure and the key bacterial genera during the PCB removal process. Therefore, the immobilization of bacteria consortium by sodium alginate-biochar enhanced the biodegradation of PCB 118, which will provide new insights into functional microorganisms' actual application for PCB restoration.

Keywords: Bacterial consortium; Biochar; Biodegradation; Immobilization; PCB 118.