LncRNA MEG8 promotes TNF-α expression by sponging miR-454-3p in bone-invasive pituitary adenomas

Aging (Albany NY). 2021 May 19;13(10):14342-14354. doi: 10.18632/aging.203048. Epub 2021 May 19.

Abstract

There are few studies on the mechanism of pituitary adenoma (PA) destroying bone. The current study aimed to investigate the role of MEG8/miR-454-3p/TNF-α in bone-invasive pituitary adenomas (BIPAs). In this study, we report that lncRNA MEG8 and TNF-α are upregulated in BIPA tissues while miR-454-3p is downregulated, which is associated with poor progression-free survival (PFS). Functional assays revealed the role of up-regulated MEG8 and down-regulated miR-454-3p in promoting bone destruction. Mechanistically, MEG8 promotes TNF-α expression by sponging miR-454-3p, which ultimately leads to the occurrence of bone destruction. The mechanism is confirmed in vivo and in vitro. Therefore, our data illustrated a new regulatory mechanism of MEG8/miR-454-3p/TNF-α in BIPAs. It may provide a useful strategy for diagnosis and treatment for BIPA patients.

Keywords: BIPAs; TNF-α; ceRNAs; lncRNA MEG8; miR-454-3p.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoma / genetics*
  • Adenoma / pathology*
  • Adult
  • Aged
  • Animals
  • Base Sequence
  • Bone and Bones / pathology*
  • Cell Line, Tumor
  • Disease Progression
  • Down-Regulation / genetics
  • Female
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mice, Nude
  • MicroRNAs / genetics
  • MicroRNAs / metabolism*
  • Middle Aged
  • Models, Biological
  • Neoplasm Invasiveness
  • Pituitary Neoplasms / genetics*
  • Pituitary Neoplasms / pathology*
  • Prognosis
  • RAW 264.7 Cells
  • RNA, Long Noncoding / genetics
  • RNA, Long Noncoding / metabolism*
  • Tumor Necrosis Factor-alpha / metabolism*
  • Up-Regulation / genetics

Substances

  • MIRN454 microRNA, human
  • MicroRNAs
  • RNA, Long Noncoding
  • Tumor Necrosis Factor-alpha