A facile SERS strategy to detect glucose utilizing tandem enzyme activities of Au@Ag nanoparticles

Xuemin Xia; Yijin Weng; Lei Zhang; Ruyi Tang; Xia Zhang

doi:10.1016/j.saa.2021.119889

A facile SERS strategy to detect glucose utilizing tandem enzyme activities of Au@Ag nanoparticles

Spectrochim Acta A Mol Biomol Spectrosc. 2021 Oct 5:259:119889. doi: 10.1016/j.saa.2021.119889. Epub 2021 Apr 30.

Authors

Xuemin Xia¹, Yijin Weng¹, Lei Zhang¹, Ruyi Tang¹, Xia Zhang²

Affiliations

¹ School of Materials Engineering, Shanghai University of Engineering Science, Shanghai 201620, China.
² School of Materials Engineering, Shanghai University of Engineering Science, Shanghai 201620, China. Electronic address: zhangxia@sues.edu.cn.

PMID: 34015600
DOI: 10.1016/j.saa.2021.119889

Abstract

Surface-Enhanced Raman Scattering (SERS) is a powerful analysis technology, attracting more and more attention due to its high sensitivity and selectivity. Herein, we report a simple seed-mediated method to synthesize Au@Ag nanoparticles (NPs) as a multifunctional biosensor for the label-free detection of hydrogen peroxide (H₂O₂) and glucose by SERS. Au@Ag NPs, as an ultrasensitive SERS substrate, show the dual activities (peroxidase-like and GOx-like activities). Under the condition of pH 4.0 NaAc buffer solution, the glucose and H₂O can be catalyzed by Au@Ag NPs to produce glucose acid and H₂O₂, and then H₂O₂ can oxidize 3,3',5,5'-tetramethylbenzidine (TMB) to form a blue oxidation product oxidic TMB (oxTMB) which exhibits strong SERS signals at 1188, 1330, 1605 cm^-1. Thus, we have developed a new SERS strategy for analysis of glucose with a detection limit of 5 × 10^-¹⁰molL^-1, suggesting that Au@Ag NPs have the potential for biosensor, immunoassay and medical treatment.

Keywords: Au@Ag nanoparticles; Glucose; H(2)O(2); Surface-Enhanced Raman Scattering; TMB.

MeSH terms

Glucose
Gold
Hydrogen Peroxide
Metal Nanoparticles*
Silver*
Spectrum Analysis, Raman

Substances

Silver
Gold
Hydrogen Peroxide
Glucose