Effects of an organic carbon source on the coupling of sulfur(thiosulfate)-driven denitration with Anammox process

Zihe Liu; Wenmin Lin; Qijin Luo; Yuancai Chen; Yongyou Hu

doi:10.1016/j.biortech.2021.125280

Effects of an organic carbon source on the coupling of sulfur(thiosulfate)-driven denitration with Anammox process

Bioresour Technol. 2021 Sep:335:125280. doi: 10.1016/j.biortech.2021.125280. Epub 2021 May 15.

Authors

Zihe Liu¹, Wenmin Lin¹, Qijin Luo², Yuancai Chen³, Yongyou Hu¹

Affiliations

¹ Ministry of Education Key Laboratory of Pollution Control and Ecological Remediation for Industrial Agglomeration Area, School of Environment and Energy, State Key Laboratory of Pulp and Paper Engineering, South China University of Technology Guangzhou, 510006, China.
² Ministry of Education Key Laboratory of Pollution Control and Ecological Remediation for Industrial Agglomeration Area, School of Environment and Energy, State Key Laboratory of Pulp and Paper Engineering, South China University of Technology Guangzhou, 510006, China; South China Institute of Environmental Sciences, MEE, Guangzhou, 510006, China.
³ Ministry of Education Key Laboratory of Pollution Control and Ecological Remediation for Industrial Agglomeration Area, School of Environment and Energy, State Key Laboratory of Pulp and Paper Engineering, South China University of Technology Guangzhou, 510006, China. Electronic address: chenyc@scut.edu.cn.

PMID: 34015567
DOI: 10.1016/j.biortech.2021.125280

Abstract

The sulfur(thiosulfate)-driven autotrophic denitrification coupled with Anammox (SDDA) process is proposed as an emerging technology for wastewater containing NH₄⁺-N and NO₃^--N. However, the influence of organic matter on the SDDA process is not fully understood. A long-term experiment has shown that a moderate organic (acetate) (＜140 mg/L COD) can accelerate the heterotrophic/autotrophic denitrification and Anammox activity, to reach as high as 92.8% ± 0.3% total nitrogen at a loading rate of 1.34 kg-N/(m³·d). Batch test results showed that Anammox made the largest contribution to the removal of nitrogen, even in an SDDA system with COD addition. Additionally, organics can promote the bioavailability of solid sulfur through reaction with sulfide to form polysulfides, which increased nitrite accumulation to forward Anammox process. Sulfur-oxidizing bacteria (e.g., Thiobacillus and Denitratisoma) coexisted with Anammox bacteria (e.g., Ca. Brocadia and Ca. Kuenenia) in the SDDA system despite the addition of exogenous COD.

Keywords: Anammox; Autotrophic/Heterotrophic denitrification; Organic carbon source; SDDA process; Thiobacillus denitrificans.

MeSH terms

Bioreactors
Carbon
Denitrification*
Nitrogen / analysis
Oxidation-Reduction
Sulfur
Thiosulfates*
Wastewater / analysis

Substances

Thiosulfates
Waste Water
Sulfur
Carbon
Nitrogen