We describe an Oropouche orthobunyavirus infection in a women 28 years of age in Colombia. We confirmed the diagnosis by viral isolation, quantitative reverse transcription PCR, and phylogenetic analysis of the small, medium, and large genomic segments. The virus is related to a strain isolated in Ecuador in 2016.
Keywords: Bunyaviridae; Colombia; Oropouche fever; Oropouche orthobunyavirus; arboviruses; vector-borne infections; viruses.