Targeted in utero electroporation of the ventro-temporal mouse retina

Alice Louail; Ahlem Assali; Xavier Nicol

doi:10.1016/j.xpro.2021.100516

Targeted in utero electroporation of the ventro-temporal mouse retina

STAR Protoc. 2021 May 3;2(2):100516. doi: 10.1016/j.xpro.2021.100516. eCollection 2021 Jun 18.

Authors

Alice Louail¹, Ahlem Assali², Xavier Nicol¹

Affiliations

¹ Sorbonne Université, Inserm, CNRS, Institut de la Vision, 17 rue Moreau, 75012 Paris, France.
² Sorbonne Université, Inserm, Institut du Fer à Moulin, 17 rue du Fer à Moulin, 75005 Paris, France.

Abstract

Techniques enabling DNA delivery into mouse retinal cells using in utero electroporation are available. However, these techniques target the central retina and do not enable the electroporation of the ventro-temporal retina where ipsilateral retinal ganglion cells are located. Here, we describe a protocol to specifically electroporate the ventro-temporal retina, a critical approach to manipulate ipsilaterally projecting retinal ganglion cells and contralaterally projecting neurons located in the same region of the retina. The procedure is adaptable to target other retinal quadrants. For complete details on the use and execution of this protocol, please refer to Louail et al. (2020).

Keywords: Microscopy; Model Organisms; Neuroscience.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Electroporation*
Female
Pregnancy
Retinal Ganglion Cells*