The Consumption of Energy Drinks Induces Blood-Brain Barrier Dysfunction in Wild-Type Mice

Liam Graneri; Virginie Lam; Zachary D'Alonzo; Michael Nesbit; John C L Mamo; Ryusuke Takechi

doi:10.3389/fnut.2021.668514

The Consumption of Energy Drinks Induces Blood-Brain Barrier Dysfunction in Wild-Type Mice

Front Nutr. 2021 May 3:8:668514. doi: 10.3389/fnut.2021.668514. eCollection 2021.

Authors

Liam Graneri^{1

2}, Virginie Lam^{1

3}, Zachary D'Alonzo^{1

2}, Michael Nesbit^{1

2}, John C L Mamo^{1

2}, Ryusuke Takechi^{1

2}

Affiliations

¹ Curtin Health Innovation Research Institute, Curtin University, Bentley, WA, Australia.
² Curtin Medical School, Faculty of Health Science, Curtin University, Bentley, WA, Australia.
³ School of Population Health, Faculty of Health Science, Curtin University, Bentley, WA, Australia.

Abstract

Energy drinks containing significant quantities of caffeine and sugar are increasingly consumed, particularly by adolescents and young adults. Chronic ingestion of energy drinks may potentially regulate vascular risk factors. This study investigated the effects of chronic ingestion of energy drinks on blood-brain barrier (BBB) integrity and neuroinflammation. Male C57BL/6J mice were maintained on water (control), Mother^TM (ED), sugar-free Mother^TM (sfED), or Coca Cola^TM soft drink (SD) for 13 weeks. The BBB integrity and neuroinflammation were analyzed with semi-quantitative immunofluorescent microscopy. Blood pressure, plasma inflammatory cytokine levels and blood glucose were also considered. Following 13 weeks of intervention, mice treated with ED, sfED, and SD showed significant disruption of BBB. However, marked neuroinflammation was observed only in sfED group mice. The consumption of ED and sfED significantly altered the blood pressure and plasma concentrations of inflammatory cytokines, TNF-a, IL-4, IL-6, and IL-10, and both increased plasma glucose. Correlation analyses showed significant associations between BBB dysfunction and hypotension, hyperglycaemia and cytokine dyshomeostasis. The intake of energy drink, particularly the sugar free formulation, may compromise the integrity of BBB and induce neuroinflammation via hypotension, hyperglycaemia and inflammatory pathways.

Keywords: blood-brain barrier; energy drinks; epithelial function; metabolic syndrome; neurovascular integrity.