Quercetin prevents myocardial infarction adverse remodeling in rats by attenuating TGF-β1/Smad3 signaling: Different mechanisms of action

Ghadeer M Albadrani; Mona N BinMowyna; May N Bin-Jumah; Gehan El-Akabawy; Hussain Aldera; Ammar M Al-Farga

doi:10.1016/j.sjbs.2021.02.007

Quercetin prevents myocardial infarction adverse remodeling in rats by attenuating TGF-β1/Smad3 signaling: Different mechanisms of action

Saudi J Biol Sci. 2021 May;28(5):2772-2782. doi: 10.1016/j.sjbs.2021.02.007. Epub 2021 Feb 13.

Authors

Ghadeer M Albadrani¹, Mona N BinMowyna², May N Bin-Jumah¹, Gehan El-Akabawy^{3

4}, Hussain Aldera⁵, Ammar M Al-Farga⁶

Affiliations

¹ Department of Biology, College of Science, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi Arabia.
² College of Applied Medical Sciences, Shaqra University, Shaqra, Saudi Arabia.
³ Department of Basic Sciences, College of Medicine, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi Arabia.
⁴ Department of Anatomy and Embryology, Faculty of Medicine, Menoufia University, Menoufia, Egypt.
⁵ Department of Basic Medical Sciences, College of Medicine, King Saud bin Abdulaziz University for Health Sciences (KSAU-HS), Riyadh, Saudi Arabia.
⁶ Biochemistry Department, College of Sciences, University of Jeddah, Jeddah, Saudi Arabia.

Abstract

This study investigated the anti-remodeling and anti-fibrotic and effect of quercetin (QUR) in the remote non-infarcted of rats after myocardial infarction (MI). Rats were divided as control, control + QUR, MI, and MI + QUR. MI was introduced to the rats by ligating the eft anterior descending (LAD) coronary artery. All treatments were given for 30 days, daily. QUR persevered the LV hemodynamic parameters and prevented remote myocardium damage and fibrosis. Also, QUR supressed the generation of ROS, increased the nuclear levels of Nrf2, and enhanced SOD and GSH levels in the LVs of the control and MI model rats. It also reduced angiotensin II, nuclear level/activity of the nuclear factor NF-κβ p65, and protein expression of TGF-β1, α-SMA, and total/phospho-smad3 in the LVs of both groups. Concomitantly, QUR upregulated LV smad7 and BMP7. In conclusion, QUR prevents MI-induced LV remodeling by antioxidant, anti-inflammatory, and anti-fibroticα effects mediated by ROS scavenging, suppressing NF-κβ, and stimulating Nrf-2, Smad7, and BMP7.

Keywords: BMPs; Fibrosis; Heart; Quercetin; Rats; Remodeling; Smad; TGF-β1.