Relation between lignin molecular profile and fungal exo-proteome during kraft lignin modification by Trametes hirsuta LE-BIN 072

Konstantin V Moiseenko; Olga A Glazunova; Olga S Savinova; Daria V Vasina; Alexander Ya Zherebker; Natalia A Kulikova; Evgeny N Nikolaev; Tatiana V Fedorova

doi:10.1016/j.biortech.2021.125229

Relation between lignin molecular profile and fungal exo-proteome during kraft lignin modification by Trametes hirsuta LE-BIN 072

Bioresour Technol. 2021 Sep:335:125229. doi: 10.1016/j.biortech.2021.125229. Epub 2021 Apr 30.

Authors

Konstantin V Moiseenko¹, Olga A Glazunova², Olga S Savinova², Daria V Vasina², Alexander Ya Zherebker³, Natalia A Kulikova⁴, Evgeny N Nikolaev³, Tatiana V Fedorova²

Affiliations

¹ A. N. Bach Institute of Biochemistry, Research Center of Biotechnology, Russian Academy of Sciences, Leninsky Ave. 33/2, Moscow 119071, Russia. Electronic address: mr.moiseenko@gmail.com.
² A. N. Bach Institute of Biochemistry, Research Center of Biotechnology, Russian Academy of Sciences, Leninsky Ave. 33/2, Moscow 119071, Russia.
³ Skolkovo Institute of Science and Technology, Skolkovo, Moscow Region 143025, Russia.
⁴ A. N. Bach Institute of Biochemistry, Research Center of Biotechnology, Russian Academy of Sciences, Leninsky Ave. 33/2, Moscow 119071, Russia; Department of Soil Science, Lomonosov Moscow State University, Moscow 119991, Russia.

PMID: 34010738
DOI: 10.1016/j.biortech.2021.125229

Abstract

The process of kraft lignin modification by the white-rot fungus Trametes hirsuta was investigated using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI FT-ICR MS), and groups of systematically changing compounds were delineated. In the course of cultivation, fungus tended to degrade progressively more reduced compounds and produced more oxidized ones. However, this process was not gradual - the substantial discontinuity was observed between 6th and 10th days of cultivation. Simultaneously, the secretion of ligninolytic peroxidases by the fungus was changing in a cascade manner - new isoenzymes were added to the mixture of the already secreted ones, and once new isoenzyme appeared both its relative quantity and number of isoforms increased as cultivation proceeded. It was proposed, that the later secreted peroxidases (MnP7 and MnP1) possess higher substrate affinity for some phenolic compounds and act in more specialized manner than the early secreted ones (MnP5 and VP2).

Keywords: (-)ESI FT-ICR MS; Exoproteome; Kraft lignin; Peroxidases; Wood rotting fungi.

MeSH terms

Lignin*
Peroxidases
Polyporaceae
Proteome
Trametes*

Substances

Proteome
Kraft lignin
Lignin
Peroxidases

Supplementary concepts

Trametes hirsuta