Silicon carbide nanoparticles electrospun nanofibrous enzymatic glucose sensor

Kavyashree Puttananjegowda; Arash Takshi; Sylvia Thomas

doi:10.1016/j.bios.2021.113285

Silicon carbide nanoparticles electrospun nanofibrous enzymatic glucose sensor

Biosens Bioelectron. 2021 Aug 15:186:113285. doi: 10.1016/j.bios.2021.113285. Epub 2021 Apr 30.

Authors

Kavyashree Puttananjegowda¹, Arash Takshi², Sylvia Thomas³

Affiliations

¹ Department of Electrical Engineering, University of South Florida, Tampa, Florida, USA. Electronic address: kavyashreep@usf.edu.
² Department of Electrical Engineering, University of South Florida, Tampa, Florida, USA. Electronic address: atakshi@usf.edu.
³ Department of Electrical Engineering, University of South Florida, Tampa, Florida, USA. Electronic address: sylvia@usf.edu.

PMID: 34004507
DOI: 10.1016/j.bios.2021.113285

Abstract

This paper presents an electrospun-nanofibrous-membrane (ENFM) of silicon carbide nanoparticles (SiCNPs) with a conductive polymer (CP) for an electrochemical enzymatic glucose sensor. The surface area of a fiber matrix is a key physical property of a nanofiber membrane for enzyme binding. It is found that glucose sensing electrodes, having a SiCNPs-ENFM nanostructure, show enhanced binding of glucose oxidase (GO_x) enzyme within the fibrous membrane. Morphological characterization of SiCNPs based ENFM was performed by using scanning electron microscopy (SEM) and using transmission electron microscopy (TEM) for SiC nanoparticles. The electrochemical analysis of SiCNPs-ENFM electrode was conducted by using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and chronoamperometry (CA) methods. Glucose concentration was detected at +0.6 V in a 5 mM potassium ferricyanide electrolyte. SiCNPs-ENFM based glucose electrodes show a detection range from 0.5 mM to 20 mM concentration with the sensitivity of 30.75 μA/mM cm² and the detection limit was 0.56 μM. The lower change in current response for SiCNPs-ENFM based glucose sensing electrodes was observed for a 50 day period.

Keywords: Biosensor; Conductive polymer; Electrochemical; Electrospun nanofibrous membrane; Glucose oxidase enzyme; Silicon carbide nanoparticles.

MeSH terms

Biosensing Techniques*
Carbon Compounds, Inorganic
Electrochemical Techniques
Electrodes
Enzymes, Immobilized
Glucose
Glucose Oxidase
Nanofibers*
Nanoparticles*
Silicon Compounds

Substances

Carbon Compounds, Inorganic
Enzymes, Immobilized
Silicon Compounds
Glucose Oxidase
Glucose
silicon carbide