Phenotypic analysis of a transgenic Drosophila model of Alzheimer's amyloid-β toxicity

Michiko Sekiya; Koichi M Iijima

doi:10.1016/j.xpro.2021.100501

Phenotypic analysis of a transgenic Drosophila model of Alzheimer's amyloid-β toxicity

STAR Protoc. 2021 Apr 29;2(2):100501. doi: 10.1016/j.xpro.2021.100501. eCollection 2021 Jun 18.

Authors

Michiko Sekiya^{1

2}, Koichi M Iijima^{1

2}

Affiliations

¹ Department of Neurogenetics, Center for Development of Advanced Medicine for Dementia, National Center for Geriatrics and Gerontology, Obu, Aichi 474-8511, Japan.
² Department of Experimental Gerontology, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya 467-8603, Japan.

Abstract

For decades, the fruit fly Drosophila melanogaster has been an efficient genetic model to investigate many aspects of human neurodegenerative diseases. Through genetic and pharmacologic approaches, these studies have revealed the molecular mechanisms underlying disease pathogenesis and provided therapeutic implications. Here, we describe a protocol for assessing Alzheimer's disease-related amyloid-β toxicity in a transgenic fly model through biochemical, histological, and behavioral analyses. We also discuss the advantages and limitations of our protocols. For complete details on the use and execution of this protocol, please refer to Wang et al. (2021).

Keywords: Behavior; Microscopy; Model Organisms; Neuroscience.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alzheimer Disease* / genetics
Alzheimer Disease* / metabolism
Amyloid beta-Peptides* / genetics
Amyloid beta-Peptides* / metabolism
Animals
Animals, Genetically Modified / genetics
Animals, Genetically Modified / metabolism
Disease Models, Animal
Drosophila melanogaster
Humans

Substances

Amyloid beta-Peptides