Dissection and surgical approaches to the mouse jugular-nodose ganglia

Wenfei Han; Ivan E de Araujo

doi:10.1016/j.xpro.2021.100474

Dissection and surgical approaches to the mouse jugular-nodose ganglia

STAR Protoc. 2021 Apr 20;2(2):100474. doi: 10.1016/j.xpro.2021.100474. eCollection 2021 Jun 18.

Authors

Wenfei Han¹, Ivan E de Araujo^{1

2

3}

Affiliations

¹ Nash Family Department of Neuroscience, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
² Pharmacology and Therapeutics Discovery Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
³ Diabetes, Obesity and Metabolism Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.

Abstract

The jugular-nodose ganglia contain the sensory peripheral neurons of the vagus nerve, linking visceral organs to the medulla oblongata. Accessing these ganglia in smaller animals without damaging the vascular and neural structures may be challenging, as ganglionic fibers imbed deeply into the carotid sheath, and vagal parasympathetic fibers cross through the interior of the ganglia. We describe a practical protocol for locating and accessing the mouse jugular-nodose ganglia in vivo, including instructions for intraganglionic injections and postperfusion dissection. For complete details on the use and execution of this protocol, please refer to Han et al. (2018).

Keywords: Metabolism; Microscopy; Neuroscience.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Dissection / methods*
Female
Jugular Foramina / innervation
Male
Mice
Nodose Ganglion* / anatomy & histology
Nodose Ganglion* / surgery

Grants and funding

R01 DC014859/DC/NIDCD NIH HHS/United States