Bacillus amyloliquefaciens CECT 5940 improves performance and gut function in broilers fed different levels of protein and/or under necrotic enteritis challenge

Kosar Gharib-Naseri; Juliano C P Dorigam; Kiran Doranalli; Natalie Morgan; Robert A Swick; Mingan Choct; Shu-Biao Wu

doi:10.1016/j.aninu.2020.05.004

Bacillus amyloliquefaciens CECT 5940 improves performance and gut function in broilers fed different levels of protein and/or under necrotic enteritis challenge

Anim Nutr. 2021 Mar;7(1):185-197. doi: 10.1016/j.aninu.2020.05.004. Epub 2020 Aug 23.

Authors

Kosar Gharib-Naseri¹, Juliano C P Dorigam², Kiran Doranalli², Natalie Morgan¹, Robert A Swick¹, Mingan Choct³, Shu-Biao Wu¹

Affiliations

¹ School of Environmental and Rural Science, University of New England, Armidale, NSW 2351, Australia.
² Evonik Nutrition & Care GmbH, Rodenbacher Chaussee 4, 63457 Hanau-Wolfgang, Germany.
³ University of New England, Armidale 2351, Australia.

Abstract

Two studies were conducted to investigate the effect of Bacillus amyloliquefaciens CECT 5940 (BA) as a probiotic on growth performance, amino acid digestibility and bacteria population in broiler chickens under a subclinical necrotic enteritis (NE) challenge and/or fed diets with different levels of crude protein (CP). Both studies consisted of a 2 × 2 factorial arrangement of treatments with 480 Ross 308 mix-sexed broiler chickens. In study 1, treatments included 1) NE challenge (+/-), and 2) BA (1.0 × 10⁶ CFU/g of feed) supplementation (+/-). In study 2, all birds were under NE challenge, and treatments were 1) CP level (Standard/Reduced [2% less than standard]) and 2) BA (1.0 × 10⁶ CFU/g of feed) supplementation (+/-). After inducing NE infection, blood samples were taken on d 16 for uric acid evaluation, and cecal samples were collected for bacterial enumeration. In both studies, ileal digesta was collected on d 35 for nutrient digestibility evaluation. In study 1, the NE challenge reduced body weight gain (BWG), supressed feed conversion ratio (FCR) and serum uric acid levels (P < 0.001). Supplementation of BA increased BWG (P < 0.001) and reduced FCR (P = 0.043) across dietary treatments, regardless of challenge. Bacillus (P = 0.030) and Ruminococcus (P = 0.029) genomic DNA copy numbers and concentration of butyrate (P = 0.017) were higher in birds fed the diets supplemented with BA. In study 2, reduced protein (RCP) diets decreased BWG (P = 0.010) and uric acid levels in serum (P < 0.001). Supplementation of BA improved BWG (P = 0.001) and FCR (P = 0.005) and increased Ruminococcus numbers (P = 0.018) and butyrate concentration (P = 0.033) in the ceca, regardless of dietary CP level. Further, addition of BA reduced Clostridium perfringens numbers only in birds fed with RCP diets (P = 0.039). At d 35, BA supplemented diets showed higher apparent ileal digestibility of cystine (P = 0.013), valine (P = 0.020), and lysine (P = 0.014). In conclusion, this study suggests positive effects of BA supplementation in broiler diets via modulating gut microflora and improving nutrient uptake.

Keywords: Bacillus amyloliquefaciens; Bacteria population; Growth performance; Gut function; Necrotic enteritis; Reduced protein diet.