Biological Effect of Silver-modified Nanostructured Titanium Dioxide in Cancer

Abstract

Background/aim: Nanomedicine is a promising scientific field that exploits the unique properties of innovative nanomaterials, providing alternative solutions in diagnostics, prevention and therapeutics. Titanium dioxide nanoparticles (TiO₂ NPs) have a great spectrum of photocatalytic antibacterial and anticancer applications. The chemical modification of TiO₂ optimizes its bioactive performance. The aim of this study was the development of silver modified NPs (Ag/TiO₂ NPs) with anticancer potential.

Materials and methods: Ag/TiO₂ NPs were prepared through the sol-gel method, were fully characterized and were tested on cultured breast cancer epithelial cells (MCF-7 and MDA-MB-231). The MTT colorimetric assay was used to estimate cellular viability. Western blot analysis of protein expression along with a DNA-laddering assay were employed for apoptosis detection.

Results and conclusion: We show that photo-activated Ag/TiO₂ NPs exhibited significant cytotoxicity on the highly malignant MDA-MB-231 cancer cells, inducing apoptosis, while MCF-7 cells that are characterized by low invasive properties were unaffected under the same conditions.

Keywords: Nanomedicine; anticancer properties; apoptosis; cytotoxicity; nanostructured titanium dioxide; photocatalysis; silver-modified titanium dioxide.