Genotypic diversity of 17 cacti species and application to biosynthesis of gold nanoparticles

Raha Soltani; Amin Baghizadeh; Hassan Karimi-Maleh; Naser Farrokhi

doi:10.1016/j.saa.2021.119909

Genotypic diversity of 17 cacti species and application to biosynthesis of gold nanoparticles

Spectrochim Acta A Mol Biomol Spectrosc. 2021 Oct 5:259:119909. doi: 10.1016/j.saa.2021.119909. Epub 2021 May 5.

Authors

Raha Soltani¹, Amin Baghizadeh², Hassan Karimi-Maleh³, Naser Farrokhi⁴

Affiliations

¹ Department of Biotechnology, Graduate University of Advanced Technology, Kerman, Iran. Electronic address: sol.raha2590@gmail.com.
² Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran. Electronic address: amin_4156@yahoo.com.
³ School of Resources and Environment, University of Electronic Science and Technology of China, P.O. Box 611731, Xiyuan Ave, Chengdu, PR China; Department of Chemical Engineering and Energy, Laboratory of Nanotechnology, Quchan University of Technology, Quchan, Iran; Department of Chemical Sciences (formerly Department of Applied Chemistry), University of Johannesburg, P.O. Box 17011, Doornfontein Campus, Johannesburg 2028, South Africa. Electronic address: hassan@uestc.edu.cn.
⁴ Department of Cell & Molecular Biology, Faculty of Life Sciences & Biotechnology, Shahid Beheshti University, Tehran, Iran.

PMID: 33992890
DOI: 10.1016/j.saa.2021.119909

Abstract

The genotypic diversity of 17 cacti species were examined and grouped in four clusters using seven inter simple sequence repeat (ISSR) markers. Group representatives (five species) were chosen for AuNPs synthesis in the cacti syrups. To synthesize the Gold nanoparticles (AuNPs), reducing and capping potential of five species of cacti rich in the polyphenolics were explored. Based on the synthesized AuNPs traits (concentration, pH, temperature, and synthesis time), Opuntia pycnacantha with the highest absorption peak at 540 nm was chosen for further characterizations. Varieties of diffraction peaks confirmed the successful synthesis of AuNPs. AuNPs functionalization with the phenolic compounds was confirmed by Fourier transform infrared (FTIR) spectroscopy. At the optimum conditions (pH = 5.0 and T = 60 °C), both dynamic light scattering (DLS) and transmission electron microscopy (TEM) revealed more than 87% of AuNPs to be 2.5 nm in size with Zeta potential to be equal to -19.9 mV.

Keywords: Biosynthesis; Cactus; Genetic diversity; Gold nanoparticles; Polyphenols.

MeSH terms

Anti-Bacterial Agents
Cactaceae*
Gold
Green Chemistry Technology
Metal Nanoparticles*
Particle Size
Plant Extracts
Spectroscopy, Fourier Transform Infrared

Substances

Anti-Bacterial Agents
Plant Extracts
Gold