A review of NMR analysis in polysaccharide structure and conformation: Progress, challenge and perspective

Hao-Ying-Ye Yao; Jun-Qiao Wang; Jun-Yi Yin; Shao-Ping Nie; Ming-Yong Xie

doi:10.1016/j.foodres.2021.110290

A review of NMR analysis in polysaccharide structure and conformation: Progress, challenge and perspective

Food Res Int. 2021 May:143:110290. doi: 10.1016/j.foodres.2021.110290. Epub 2021 Mar 9.

Authors

Hao-Ying-Ye Yao¹, Jun-Qiao Wang², Jun-Yi Yin³, Shao-Ping Nie⁴, Ming-Yong Xie⁵

Affiliations

¹ State Key Laboratory of Food Science and Technology, China-Canada Joint Lab of Food Science and Technology (Nanchang), Nanchang University, 235 Nanjing East Road, Nanchang 330047, China. Electronic address: ncuskyaohaoyingye@163.com.
² State Key Laboratory of Food Science and Technology, China-Canada Joint Lab of Food Science and Technology (Nanchang), Nanchang University, 235 Nanjing East Road, Nanchang 330047, China. Electronic address: ncuskwangjunqiao@163.com.
³ State Key Laboratory of Food Science and Technology, China-Canada Joint Lab of Food Science and Technology (Nanchang), Nanchang University, 235 Nanjing East Road, Nanchang 330047, China. Electronic address: yinjy@ncu.edu.cn.
⁴ State Key Laboratory of Food Science and Technology, China-Canada Joint Lab of Food Science and Technology (Nanchang), Nanchang University, 235 Nanjing East Road, Nanchang 330047, China. Electronic address: spnie@ncu.edu.cn.
⁵ State Key Laboratory of Food Science and Technology, China-Canada Joint Lab of Food Science and Technology (Nanchang), Nanchang University, 235 Nanjing East Road, Nanchang 330047, China; National R&D Center for Freshwater Fish Processing, Jiangxi Normal University, Nanchang, Jiangxi 330022, China. Electronic address: myxie@ncu.edu.cn.

PMID: 33992390
DOI: 10.1016/j.foodres.2021.110290

Abstract

Nuclear magnetic resonance (NMR) has been widely used as an analytical chemistry technique to investigate the molecular structure and conformation of polysaccharides. Combined with 1D spectra, chemical shifts and coupling constants in both homo- and heteronuclear 2D NMR spectra are able to infer the linkage and sequence of sugar residues. Besides, NMR has also been applied in conformation, quantitative analysis, cell wall in situ, degradation, polysaccharide mixture interaction analysis, as well as carbohydrates impurities profiling. This review summarizes the principle and development of NMR in polysaccharides analysis, and provides NMR spectra data collections of some common polysaccharides. It will help to promote the application of NMR in complex polysaccharides of biochemical interest, and provide valuable information on commercial polysaccharide products.

Keywords: Application; Conformation; NMR; Polysaccharide; Structure.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Carbohydrate Sequence
Magnetic Resonance Spectroscopy
Molecular Conformation
Nuclear Magnetic Resonance, Biomolecular
Polysaccharides*

Substances

Polysaccharides