Mediterraneibacter catenae SW178 sp. nov., an intestinal bacterium of feral chicken

Supapit Wongkuna; Sudeep Ghimire; Surang Chankhamhaengdecha; Tavan Janvilisri; Joy Scaria

doi:10.7717/peerj.11050

Mediterraneibacter catenae SW178 sp. nov., an intestinal bacterium of feral chicken

PeerJ. 2021 Apr 27:9:e11050. doi: 10.7717/peerj.11050. eCollection 2021.

Authors

Supapit Wongkuna^{1

2}, Sudeep Ghimire^{2

3}, Surang Chankhamhaengdecha⁴, Tavan Janvilisri¹, Joy Scaria^{2

3}

Affiliations

¹ Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand.
² Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, South Dakota, United States.
³ South Dakota Center for Biologics Research and Commercialization, Brookings, South Dakota, United States.
⁴ Department of Biology, Faculty of Science, Mahidol University, Bangkok, Thailand.

Abstract

A Gram-positive, coccobacillus, white raised and circular with an entire edge colony, and obligately anaerobic bacterium, strain SW178 was isolated from the cecum content of feral chickens in Brookings, South Dakota, USA. The most closely related strain based on 16S rRNA gene sequence analysis of strain SW178 was Mediterraneibacter torques ATCC 27756^T (Ruminococcus torques ATCC 27756^T) with 96.94% similarity. The genome of strain SW178 is 3.18 Mbp with G+C content of 46.9 mol%. The optimal temperature and pH for growth in modified brain heart infusion (BHI-M) medium were 45 °C and pH 7.5, respectively. The sole carbon sources of the strain were dextrin, L-fucose, D-galacturonic, α-D-glucose, L-rhamnose and D-sorbitol. The primary cellular fatty acids were C_{14 : 0}, C_{16 : 0} and C_{16 : 0} dimethyl acetal (DMA). Based on the genotypic and phenotypic comparison, we proposed that strain SW178 belong to the genus Mediterraneibacter in the family Lachnospiraceae as a novel species, in which the name Mediterraneibacter catenae is proposed. The type strain is SW178 (= DSM 109242^T = CCOS 1886^T).

Keywords: Culturomics; Feral chicken; Gut microbiome; Mediterraneibacter catenae; Novel species; Taxonogenomic.

Grants and funding

This work was supported by the USDA National Institute of Food and Agriculture, Hatch projects SD00H532-14 and SD00R540-15, and a grant from the South Dakota Governor’s Office of Economic Development awarded to Joy Scaria, and Mahidol University to Surang Chankhamhaengdecha and Tavan Janvilisri. Supapit Wongkuna received a fellowship from the Science Achievement Scholarship of Thailand. There was no additional external funding received for this study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.