RNA polymerase I subunit 12 plays opposite roles in cell proliferation and migration

Biochem Biophys Res Commun. 2021 Jun 30:560:112-118. doi: 10.1016/j.bbrc.2021.04.091. Epub 2021 May 10.

Abstract

RNA polymerase I (Pol I) is responsible for the synthesis of the majority of ribosomal RNA molecules in eukaryotes. Pol I subunit 12 (RPA12) is involved in the transcriptional termination and lipid metabolism in yeast. However, its role in human cells hasn't been investigated so far. Here, we show that RPA12 is present in the nucleolus and nucleoplasm of HeLa cells. RPA12 can act as a positive factor to regulate Pol I-mediated transcription and the proliferation of 293T and HeLa cells. Unexpectedly, RPA12 can repress HeLa cell migration, indicating that RPA12 plays opposite roles in cell proliferation and migration. This study provides a novel insight into the role of RPA12 in human cells.

Keywords: Cell migration; Cell proliferation; RNA polymerase I; RPA12; rRNA transcription.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Movement*
  • Cell Nucleolus / chemistry
  • Cell Nucleus / chemistry
  • Cell Proliferation*
  • DNA-Binding Proteins / analysis
  • DNA-Binding Proteins / physiology*
  • Gene Expression Regulation
  • HEK293 Cells
  • HeLa Cells
  • Humans
  • RNA Polymerase I / metabolism
  • Transcription, Genetic

Substances

  • DNA-Binding Proteins
  • POLR1H protein, human
  • RNA Polymerase I