Black phosphorus (BP) is a typical two-dimensional (2D) layered material with strong in-plane anisotropy and large birefringence, making it possible to manipulate the light field with atomically controlled devices for various optoelectronic and photonic applications-for instance, atomic thickness waveplates. The twist angle in twisted black phosphorus (TBP) can be presented as a new tunable dimension to control BP's optical anisotropy. Here, we report a large and tunable optical rotation effect in TBP, the result of regulating the twist angle and BP thickness. To accurately study the optical rotation and the impact of the twist angle, we developed a new method to prepare TBP. A lab-made polarimeter microscope was used to visualize the optical rotation mapping of TBP. A large polarization-plane rotation (PORA) of 0.49° per atomic layer was observed from an air/BP/SiO2/Si Fabry-Pérot cavity at 600 nm, an order of magnitude higher than the PORA of 0.05° per atomic layer reported earlier. For the same thickness, the PORA of TBP can be tuned from 0.48° to 7.75° based on the twist angle from 0° to 90°. Our work provides an efficient method to investigate the anisotropy of 2D materials and their heterojunctions. TBP could help us design novel optical and optoelectronic devices such as tunable nanoscale polarization controllers.