Screening extracts from the fermentation broth of a collection of actinomycetes can be performed conveniently on a microtiter plate format. Here we describe a method suited for the screening of antibacterial extracts or compounds against Gram-positive and Gram-negative aerobic, non-fastidious bacteria, Escherichia coli and Kocuria rhizophila. Both bacteria have been chosen to minimize the need for containment as they belong to risk group 1.
Keywords: Antibacterial bioassay; Escherichia coli ATCC 25922; Kocuria rhizophila ATCC 9341.