Effect of alfalfa (Medicago sativa L.) saponins on meat color and myoglobin reduction status in the longissimus thoracis muscle of growing lambs

Anim Sci J. 2021 Jan-Dec;92(1):e13556. doi: 10.1111/asj.13556.

Abstract

The effect of alfalfa saponins (AS) supplementation on the meat quality especially the color for growing lamb was investigated. Fifty Hu male lambs with body weights (BW, 19.21 ± 0.45 kg) were divided into five groups and supplemented AS with 0, 500, 1,000, 2,000, and 4,000 mg/kg of dietary dry matter intake. After 90 days, all lambs were slaughtered. The longissimus thoracis muscle in lamb displayed significant changes in the content of intramuscular fat, especially n-3 polyunsaturated fatty acids, and drip loss within AS treatment (p < .05) between control and treatments groups. Redness (a*) significantly improved in both 0-day and 7-day storage with the AS supplementation coupled with the percentage of met-myoglobin reduction (p < .05). The redness (a*) change may result from improved met-myoglobin reducing activity, antioxidant enzymes, lactate dehydrogenase, and succinate dehydrogenase (p < .05) by AS supplementation in muscle. These enzymes may help to protect mitochondria function and reduce met-myoglobin, which bring a bright and red meat color.

Keywords: alfalfa saponins; antioxidant traits; growing lambs; meat quality; myoglobin reducing activity.

MeSH terms

  • Adipose Tissue / metabolism
  • Animal Nutritional Physiological Phenomena*
  • Animals
  • Color*
  • Diet / veterinary*
  • Dietary Supplements*
  • Fatty Acids, Omega-3 / metabolism
  • Food Quality*
  • Food Storage / methods
  • Male
  • Meat* / analysis
  • Medicago sativa / chemistry*
  • Muscle, Skeletal / metabolism*
  • Myoglobin / metabolism*
  • Saponins / administration & dosage*
  • Saponins / isolation & purification
  • Sheep / growth & development*
  • Sheep / metabolism*
  • Time Factors

Substances

  • Fatty Acids, Omega-3
  • Myoglobin
  • Saponins