Phylogenetic relationships and characterization of the complete chloroplast genome of Rosa sterilis

Huiqing Yan; Yanjing Liu; Zongmin Wu; Yin Yi; Xiaolong Huang

doi:10.1080/23802359.2021.1915200

Phylogenetic relationships and characterization of the complete chloroplast genome of Rosa sterilis

Mitochondrial DNA B Resour. 2021 Apr 26;6(4):1544-1546. doi: 10.1080/23802359.2021.1915200.

Authors

Huiqing Yan¹, Yanjing Liu^{2

3}, Zongmin Wu^{2

3}, Yin Yi^{2

3}, Xiaolong Huang^{2

3}

Affiliations

¹ School of Life Sciences, Guizhou Normal University, Guiyang, PR China.
² Key Laboratory of State Forestry Administration on Biodiversity Conservation in Mountainous Karst Area of Southwestern China, Guizhou Normal University, Guiyang, PR China.
³ Key Laboratory of Plant Physiology and Development Regulation, Guizhou Normal University, Guiyang, PR China.

Abstract

Rosa sterilis is an economically and important fruit that is extensively grown in Southwestern China. In this study, we determined the complete chloroplast genome of R. sterilis using high-throughput Illumina sequencing. The chloroplast genome of R. sterilis is 156,561 bp in size, containing a large single-copy region (LSC)(85,701 bp), a small single-copy region (SSC) (18,746 bp), and a pair of inverted repeat (IR) regions (each one of 26,057 bp). The overall GC content of the chloroplast genome is 37.23%, while the corresponding values of GC contents of the LSC, SSC, and IR regions are 35.20%, 31.37%, and 42.70%, respectively. The chloroplast genome of R. sterilis contains 130 genes, including 84 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. The phylogenetic maximum-likelihood tree revealed that Rosa chinensis or Rosa chinensis var. spontanea is the closest related to R. sterilis in the phylogenetic relationship. This complete chloroplast genome can be further used for genomic studies, evolutionary analyses, and genetic engineering studies of the family Rosaceae.

Keywords: Rosa sterilis; Rosaceae; chloroplast genome; phylogenetic relationship.

Grants and funding

This work was supported by grants from the National Natural Science Foundation of China [Grant No. 32060587, 31660554 and 31660046], The Joint Fund of the National Natural Science Foundation of China and the Karst Science Research Center of Guizhou province [Grant No. U1812401], Guizhou Normal University Dr. Scientific Research Fund [11904-0514156 and 11904-0514157], Guizhou Educational project Qianjiaohe [2021]309].