Diversity and authentication of Rubus accessions revealed by complete plastid genome and rDNA sequences

Young Sang Park; Jee Young Park; Jung Hwa Kang; Wan Hee Lee; Tae-Jin Yang

doi:10.1080/23802359.2021.1911712

Diversity and authentication of Rubus accessions revealed by complete plastid genome and rDNA sequences

Mitochondrial DNA B Resour. 2021 Apr 20;6(4):1454-1459. doi: 10.1080/23802359.2021.1911712.

Authors

Young Sang Park¹, Jee Young Park¹, Jung Hwa Kang², Wan Hee Lee², Tae-Jin Yang¹

Affiliations

¹ Department of Agriculture, Forestry and Bioresources, Plant Genomics and Breeding Institute, College of Agriculture and Life Sciences, Seoul National University, Seoul, Korea.
² Hantaek Botanical Garden, Yongin, Korea.

Abstract

Complete plastid genome (plastome) and ribosomal DNA (rDNA) sequences of three Rubus accessions (two Rubus longisepalus and one R. hirsutus) were newly assembled using Illumina whole-genome sequences. Rubus longisepalus Nakai and R. longisepalus var. tozawai, described as different varieties, have identical plastomes and rDNA sequences. The plastomes are 155,957 bp and 156,005 bp and the 45S rDNA transcription unit sizes are 5809 bp and 5811 bp in R. longisepalus and R. hirsutus, respectively. The 5S rDNA transcription unit is an identical 121 bp in three Rubus accessions. We developed three DNA markers to authenticate R. longisepalus and R. hirsutus based on plastome diversity. Phylogenomic analysis revealed that the Rubus species classified as two clades and R. longisepalus, R. hirsutus, and R. chingii are the most closely related species in clade 1.

Keywords: R. hirsutus; Rubus longisepalus; chloroplast; phylogenetic tree; rDNA.

Grants and funding

This research was supported by a research grant from the KIST ORP program [BlueBell Research program 2E30650].