Evidence of viral genome linked protein of banana bract mosaic virus interaction with translational eukaryotic initiation factor 4E of plantain cv. Nendran based on yeast two hybrid system study

Chelliah Anuradha; R Selvarajan; T Jebasingh; P Sankara Naynar

doi:10.1007/s13337-021-00672-9

Evidence of viral genome linked protein of banana bract mosaic virus interaction with translational eukaryotic initiation factor 4E of plantain cv. Nendran based on yeast two hybrid system study

Virusdisease. 2021 Mar;32(1):123-130. doi: 10.1007/s13337-021-00672-9. Epub 2021 Apr 5.

Authors

Chelliah Anuradha¹, R Selvarajan¹, T Jebasingh², P Sankara Naynar²

Affiliations

¹ ICAR-National Research Centre for Banana, Thogamalai Road, Thayanur Post, Tiruchirappalli, Tamil Nadu India.
² Department of Plant Sciences, School of Biological Sciences, Madurai Kamaraj University, Madurai, Tamil Nadu India.

Abstract

Banana bract mosaic virus (BBrMV), belongs to the genus Potyvirus and it is an important viral pathogen of bananas and plantains. The eukaryotic translation initiation factor, eIF4E, and its isoform play key roles during the virus infection in plants, particularly Potyvirus. The present study was undertaken to determine the role of BBrMV-viral protein genome-linked (VPg) in virus infectivity by analyzing the interaction with the eukaryotic translation initiation factor eIF4E through yeast two-hybrid system. The results suggest that plantain cv. Nendran eIF4E plays an essential role in the initiation of the translation of capped mRNAs and its association with VPg would point to a role of the viral protein in the translation of the virus and may potentially contribute to BBrMV resistance.

Keywords: Banana bract mosaic virus; EIF4E; Plantain cv. Nendran; Viral protein genome-linked; Yeast two hybrid system.