Background: Tomato is one of the widely cultivated crops worldwide that is affected by several pests, such as fungi (Fusarium oxysoporum, Alternaria solani), bacteria (Pectobacterium carotovorum) and weeds (Cyperus iria L., Amaranthus spinosus). A growing interest has emerged for developing plant-derived pesticidal compounds to counteract these pests. One attractive alternative is to use barnyard grass (Echinochloa crus-galli), known to be widely resistant to synthetic herbicides, as a potential biopesticide compound source.
Results: Phytochemical screening of the crude extract showed that phenolic compounds were the most abundant component present in barnyard grass. The crude extract was evaluated for antifungal, antibacterial and herbicidal activities. Bioassays showed inhibition against F. oxysporum (10.73 ± 1.30%) and A. solani (20.47 ± 3.51%), the causative agent of Fusarium rot and early blight disease in tomato, respectively. Antibacterial activity against P. carotovorum gave a mean zone of inhibition (paper disc diffusion assay) of 17.00 ± 1.00 mm and an IC50 (dose-response assay) of 2.26 mg mL-1 was observed. Dose-responsive herbicidal activity on the lettuce seed germination bioassay produced an IC50 of 459.30 ppm. Selectivity studies showed inhibition towards C. iria and A. spinosus with no effect on tomato. Lastly, bioassay-guided fractionation coupled with untargeted metabolomics studies using ultra-performance liquid chromatography with diode array detection-tandem mass spectrometry mass analyses revealed loliolide and tricin as the putative metabolites present in barnyard grass.
Conclusion: To date, this is the first reported study on using barnyard grass as a potential alternative biopesticide against tomato pests such as fungi, bacteria and weeds. © 2021 Society of Chemical Industry.
Keywords: Echinochloa crus-galli; antibacterial; antifungal; herbicide; loliolide; tricin.
© 2021 Society of Chemical Industry.